Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis

For producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM ATR-FTIR spectroscopy showed that nanoparticles are homogeneously distributed onto GO sheets surface. The enzymatic preparation immobilized means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) N-hydroxysuccinimide (NHS). supported final biocatalyst (GO-MNP-Enz) high activity hydrolysis pretreated sugarcane bagasse (PSB) presented relative endoglucanase, xylanase, ?-glucosidase, ?-xylosidase activities 70%, 66%, 88%, respectively, after 10 cycles their respective substrates. also maintained approximately 50% 80% its efficiency cellulose xylan hydrolysis, being TOF (g.g?1.h?1) highest observed when compared with previous results reported literature. These findings suggest GO-MNP-Enz may be a prospective candidate applications such production.